Enhancing pre-harvest sprouting tolerance in wheat

Enhancing pre-harvest sprouting tolerance in wheat

Researchers: Belay Ayele
Keywords: Genomics Wheat
Start Date: 2019
Status: In Progress

Background:  This would be a continuation of work started under a GF2 project that was funded by WGRF and MB GF2 funding.  It was started just as we were starting up and MWBGA contributed research management as an in-kind contribution.  Lori-Ann wrote contracts and coordinated reporting to all the funders.

Lead Researcher:  Belay Ayele, University of Manitoba
Collaborators:  Dr. Curt McCartney & Mark Jordan, AAFC – Morden
Start Date:  2019
Project Length: 3 Years (2019-2023)
Project Status: APPROVED – Contracting

This project focuses on a newly developed wheat double haploid population derived from a cross between preharvest sprouting (PHS) resistant line RL4137 and PHS susceptible cultivar Roblin for which the genotypic and phenotypic studies are in progress as a collaborative project between Dr. Ayele’s lab and the labs of Dr. Mark Jordan and Dr. McCartney at AAFC-Morden. Still early discovery research moving one step closer to incorporation in a variety.  How well do new genetic traits discovered over the last 3 years express PHS tolerance in a new cross and can we use new technologies to see it earlier? The work proposed in this LOI extends the scope of the study beyond the genetic/QTL analysis to integrate genome wide RNA-sequencing (RNA-seq) and targeted metabolomics approaches to identify novel genes and molecular variants that can accelerate wheat breeding for PHS tolerance.

Objectives:  

  1. Novel genes for improving tolerance of wheat grains to preharvest sprouting.
  2. New molecular variants/markers to facilitate breeding for sprouting tolerance, and genomic resources to the public functional genomics data repository.
  3. Adapted wheat germplasm with optimum dormancy, and highly qualified personnel in the area of wheat genomics.

Total project cost:  $240, 583
MWBGA funding requested:
$124,583
Partner funding:  $116,000 CAP Ag Action MB + AAFC in-kind

Benefits to MB Producers: Early stage genetic research that includes verification of new genomics tools with potential to shorten the breeding cycle.